Page 77 - Annual Report_21-22
P. 77

Mine reclamation through phytoremediation phytoremediation of hexavalent chromium from
                 mine-overburden dump and revegetation of barren land at OMC mines, South Kaliapani,
                                                      Odisha, India

            Chromite mine overburden areas are degraded land with  undertaken during rainy season of each year (2019, 2020,
            low fertility and productivity. The high slopy overburden  2021) to facilitate the successful establishment of plants.
            land makes the site more difficult to plant the saplings.  Regular  watering  and  fertilization/biochar  treatments
            To  achieve the success, plant saplings/slips of  suitable  were planned and undertaken to protect the plants from
            species were  raised in the experimental garden of the  extreme  heat  and  water  deficit  situations.  The  project
            Bioremediation  lab  of  CSIR-IMMT,  BBSR.  In  the  project,  targets to cover 10 hectares of mine overburden area for
            about five hectares of mine overburden area were planted  phytoremediation studies and its success will establish a
            with  Lemongrass  (Cymbopogon  flexuosus)  and  Vetiver  leading  platform  for  revegetation  strategies  in  chromite
            (Chrysopogon  zizaniodes),  Palmarosa  (Cymbopogon  and other mining areas. The photographs are given below
            martini) and Napier grass (Pennisetum purpureum) species  reflecting the site and operation under the project
            in  the  first  two  years.  The  plantation  activities  were


















                   Biomaterials biodegradable polyxydroxyalkanoate based polymers from marine and
                                                  wastewater bacteria
            Through a Government of Odisha sponsored project, there   polymer quality were compared to the standard reference
            is ongoing research on bio-based and biodegradable plastic   culture of  Cupriavidus  oxalaticus.  The  GC-MS  profile  of
            like material - Polyhydroxyalkanoate (PHA) polymers. The   the polymers produced by the consortia of bacteria was
            cost of production in the current industrial process is high   also  compared to the commercially  purchased homo-
            due to several factors including the cost of carbon source   polymer of 3-hydroxybutanoic acid (PHB) which shows a
            for  microbial  growth  and  polymer  production  in  pure   peak at R  3.970. We found that the polymers produced
                                                                      t
            cultures of microbes. We aimed to mass culture bacteria in   by C. oxalaticus shows the presence of methyl esters of
            a consortium on natural waste  substrates to find a cheaper   3-hydroxybutyrate at R 3.747 and in the case of marine
                                                                                 t
            alternative source of substrates for bacterial growth ex-  microbial consortia it is at R  3.740.
                                                                                    t
            situ. We compared it to the plastic film produced by the   The GC-MS profile of the “Control” commercially available
            same  bacterial  consortium  in  a  well-defined  minerals   pure  PHB  polymer  (A)  compared  with  the  polymer
            salts  medium.  A  consortium  of  marine  bacteria  and   produced  by  C.  oxalaticus  (B)  and  marine  microbial
            wastewater  bacteria  was  developed  to  maximize  PHA   consortia (C):
            production  on  food  waste  substrates.  Their  growth  and

                                                                                           A

















            ANNUAL REPORT 2021-22                                                                               75
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