Page 79 - Annual Report_21-22

Design, fabrication and characterizing the primer derived DNA nanostructures

The Structural and molecular characteristics features These rules extend from the sequence designing to self-
make DNA the most remarkable programmable material assembly into an error-free hybridization forming stable
in the nanoscale platform. The emerging structural DNA nanostructures. The spectroscopic study characterizes
nanotechnology field exploits the addressable code the B-form of DNA with their respective minor and major
for novel structural and sequence motifs. Herein, we grooves in the DNA nanostructures. The laser scattering
demonstrate an approach that allows the use of primer system characterizes its hydrodynamic diameter and
allowing the integration of those primers’ sequences into the potential surface charge of the nanostructures. The
the oligonucleotides that self-assemble to form a planar thermal melting and the nuclease resistance report the
benzene like monomer branched DNA (bDNA) and DNA enhanced structural stability of the monomer than the
tetrahedrons. The custom primer derived oligonucleotides linear duplex DNAs. Thermodynamic studies between
are an integrated part of the functional diversity of the individual oligonucleotides confirm the formation of
primers. Each oligonucleotide is designed to recognize the DNA duplex in a sequence and directional specificity. This
sequence specific complementarity with the directional detailed report describes in the creation of monomeric
specificity. In the current study we present designing rules bDNA nanostructures with a primer derived sequences
for the construction of the specific bDNA nanostructures that self-assemble with a characteristics B-form DNA
beyond the computer aided software and trial and error duplex with enhanced structural stability for the biological
methodology and self-assembly properties. and other application purposes.

Self-assembled DNA nanostructure containing oncogenic miRNA-mediated cell

Proliferation and downregulation of FOXO1 expression
A FOXO1 transcription factor is not only limits the cell cycle miRNA sequences can selectively bind to mRNA and inhibit
progression but also promotes cell death as a tumour translation. Secondly, the downstream gene expression
suppressor protein. Though the expression of FOXO1 is of p21 and p27 are also significantly downregulated
largely examined in breast cancer, the regulation of FOXO1 in the presence of miR-bDNA nanostructures. The cell
by miRNA is yet to be explored. In the current study, proliferation activity was progressively increased in the
self-assembled branched DNA (bDNA) nanostructures presence of miR-bDNA nanostructure which confirms
containing oncogenic miRNAs were designed and the reduced tumour suppression activity of FOXO1. This
transfected to breast cancer MCF7 cell lines to decipher the finding can be explored to design novel bDNA structures
FOXO1 expression. bDNA containing oncogenic miRNA 27a, which can downregulate the tumour suppressor protein
96 and 182 synergistically downregulate the expression of in normal cells and induce cell proliferation activity to
FOXO1 in MCF7 cells. The down-regulation is evident both identify early phase markers of cancer.
in mRNA and protein levels suggesting that bDNA having
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